Detection of intra-familial transmission of shigella infection using conventional serotyping and pulsed-field gel electrophoresis
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چکیده
منابع مشابه
Pulsed-field Gel Electrophoresis for Salmonella Infection
To detect Salmonella clusters, public health laboratories perform pulsed-field gel electrophoresis (PFGE) that provides a PFGE pattern, or DNA fingerprint. If the PFGE patterns of isolates from >2 persons are indistinguishable, the responsible bacteria may be related to a common source (1–3). PulseNet is a network of public health laboratories coordinated by the Centers for Disease Control and ...
متن کاملSubtyping of Salmonella enterica isolated from humans and food animals using Pulsed-Field Gel Electrophoresis
Salmonella infections are the second leading cause of zoonotic bacterial foodborne illness. Main source of infection in human is contaminated food products. The aim of this study was sub typing isolates of Salmonella entericaobtained during our previous study byPulsed Field Gel Electrophoresis (PFGE) technique. All 46 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates...
متن کاملPulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
متن کاملPulsed field gel electrophoresis using a double-decker gel system.
Pulsed field gel electrophoresis (PFGE) is capable of resolving large DNA molecules of up to two million nucleotides and is becoming a widely used technique in DNA studies (1—3). Three factors can potentially limit PFGE in a laboratory: 1. the cost of the apparatus, 2. reproducibility of electrophoretic conditions, and 3. unusually long electrophoretic time. To alleviate some of these problems ...
متن کاملComparison of Genetic Patterns of E. coli Strains Isolated from Patients with Cystitis and Pyelonephritis, Using Pulsed Field Gel Electrophoresis
Background & Aims: Urinary tract infection (UTI) is one of the most frequently acquired bacterial infections caused by a large genetically heterogeneous group of Escherichia coli which are called uropathogenic E. coli (UPEC). Cystitis and pyelonephritis are two most common symptoms seen in patients with UTI. The genetic diversity of this organism has hampered the identification of UTI strains a...
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ژورنال
عنوان ژورنال: Epidemiology and Infection
سال: 2005
ISSN: 0950-2688,1469-4409
DOI: 10.1017/s0950268805005534